Astaxanthin dilanni ni Levure ye:
Biotechnologie ka ɲɛtaa ani Industriw ka jateminɛw
Astaxanthin ye keto-carotenoïde ye min bɛ sɔrɔ a yɛrɛ la, min lakodɔnnen don kosɛbɛ a ka farikolo tangacogo danfaralen na ani a kɛcogo caman na in...jiko, dumuni dafalanw, furaw, ani ɲɛgɛnw. I n’a fɔ suguw ka ɲininiwastaxantini nafamabɛ ka taa a fɛ ka bonya, mikrobiyɔmu biyosintɛzi-kɛrɛnkɛrɛnnenya la ni yezu ye-o bɔra kɛnɛ kan i n’a fɔ izini dilanni sira layidu talenba dɔ.
Nin barokun bɛ an...izini-lajɛba min ɲɛsinnen bɛ izini maka yeast-based astaxanthin bɔli, ka sinsin a kanbiyosintɛzi siraw, sɛnɛfɛnw sɛnɛcogo fɛɛrɛw, ani fermentation optimisation, min sinsinnen bɛ dɔnniya ɲɛtaa kan kɔsa in na.
1. Mun na Astaxanthin nafa ka bon diɲɛ sugu la
Astaxanthin nafa ka bon a ka farikolo tangacogo barikama kosɔn, a ka se ka funu kɛlɛ, ani a jɔyɔrɔ ye farikolo tangacogo dɛmɛni na, ɲɛ kɛnɛya la, ani fari lakanani na. Ni an y’a suma ni astaxanthin ye min dilannen don ni kemikali ye, .astaxanthin min bɛ sɔrɔ a yɛrɛ lanafa caman bɛ a la:
Biologique (ɲɛnamaya baara) min ka bon ka da stereochimique (stɛriyɔmu) cogoya ɲɛfɔlenw kan
Kemikɛli sintezi tolenw tɛ yen
Bioavailabilité ka fisa, a ka ca a la a bɛ sɔrɔ esterifié cogo la
O de kosɔn, astaxanthin nafama kɛra...sugandili min ka fisa dumuni, dumuni dafalanw, ani ɲɛgɛnw kɛcogo la, kɛrɛnkɛrɛnnenya la suguw la minnu bɛ sɔngɔko ni sariyaw{0}}sensitive.
2. Mun na Levure ye fɛn dilanni yɔrɔ ɲuman ye
Sigida sɔrɔyɔrɔ suguya caman cɛma, yezu bɛ bɔ kɛnɛ kan i n’a fɔ banakisɛw ka izini barikama ani min bɛ se ka sɛgɛsɛgɛ astaxanthin dilanni na k’a sababu kɛ:
Bonya teliya ani a mɔli waati kunkurunnin
Substrat baarakɛcogo ka bon
Kɛnɛ -sìgi sen kan jɛnɛya baarakɛminɛnw
Mɔni kɛcogo minnu sɛgɛsɛgɛra iziniw kɔnɔ
Yezu suguya minnu bɛ sɔrɔ tuma caman na minnu sɛgɛsɛgɛra ani minnu kɛra astaxanthin biyosintɛzi la, olu ye:
Xanthophyllomyces dendrorhous (Santhophyllomyces dendrorhous) bɛ sɔrɔ a la(Fafia rodozima) .
Saccharomyces cerevisiae (banakisɛ min bɛ wele ko Saccharomyces cerevisiae).
Yarrowia lipolytica (banakisɛfagalan min bɛ wele ko Yarrowia lipolytica).
Kluyveromyces marxianus ye banakisɛ ye
O fɛnɲɛnamaw bɛ baara Kɛ walima i n’a fɔnafamafɛnw dilannikɛlawwalima i n’a fɔjatigila minnu ye ɛntɛrinɛti yeka ɲɛsin astaxanthin siraw ma minnu tɛ kelen ye.
3. Astaxanthin Biosynthèse (Astaxanthin Biosynthèse) lajɛlen ye yezu la
Astaxanthin biosynthèse min bɛ yezu la, a ka c’a la, o bɛ taa ɲɛ santaabolo fila:
3.1 -Karotɛni (Karotɛni) sɔrɔli
O kɛcogo bɛ daminɛ ni karɔbɔni cɛmancɛlabɔli ye, yɔrɔ min na sukaro i n’a fɔ sukaro bɛ wuli ka kɛ isoprenoid ɲɛbilafɛnw ye ka tɛmɛ...mevalonate (MVA) sira fɛ, o min ye fɛn ye min bɛ bɔ yezu la. Cɛmancɛ-minɛn jɔnjɔnw i n’a fɔ IPP ani DMAPP bɛ lajɛ ka wuli ka kɛ :
Jeraniljeranili pirofosifati (GGPP) .
Fitoɛni (fitoène) ye
Likopɛni (Licopene) bɛ sɔrɔ
-Karotɛni
O dakun in de bɛ a dantigɛkabɔn bɔli sekoka ɲɛsin astaxanthin bɔli ma.
3.2 -Karotɛni jiginni ka kɛ Astaxanthin ye
-Karotɛni bɛ wuli ka kɛ astaxanthin ye ni wale fila ye minnu bɛ tugu ɲɔgɔn na:
Hydroxylation (jidɔrɔkisili) (hydroxylation).
Ketolation (ketolation) ye
KɔnɔX. dendrorhous (dendrorhous) ye, o jiginni in bɛ kɛ ni sitokɔrɔmu P450-based astaxanthin synthase system ye.
Yezu suguya minnu dilannen don ni ɛntɛrinɛti ye, jɛnɛya minnu bɛ -karotɛni hidroksilazi ni ketolazi kofɔ (a ka ca a lacrtZ yeanicrtW/BKT ye) bɛ don a la ka bɔ banakisɛ walima jirisunw na.
O kɛcogo in senfɛ, cɛmancɛ karotɛnoyiw i n’a fɔ kantaksantini walima ɛkinɛnɔni fana bɛ se ka sɔrɔ.
4. Fɛɛrɛ minnu bɛ kɛ walasa ka dɔ fara Astaxanthin sɔrɔli kan
4.1 Sɛgɛsɛgɛli ɲɛnabɔli
A ka ca a la, yezu suguya minnu bɛ sɔrɔ u yɛrɛ la, olu bɛ astaxanthin dilan ni u hakɛ ka dɔgɔn. Walasa ka baarakɛcogo ɲɛ, fɛɛrɛ caman bɛ kɛ ka caya:
Mutagenesis (Mutagenesis) sɛnɛcogo(misali la, UV, gama fiɲɛ, ARTP) .
Laboratoire adaptatif ka jiginni
Metaboliki injiniyɛri, i n'a fɔ:
Anzimu minnu bɛ dan sigi key rate-kan jirali kojugu
Sira minnu bɛ ɲɔgɔn sɔsɔ, olu dɔgɔyali (misali la, stɛroli biyosintɛzi) .
Jɛnkulu sɛgɛsɛgɛli tigitigi ni CRISPR/Cas sitɛmuw ye
O fɛɛrɛw sababu fɛ, yezu suguya minnu dilannen don ni ɛntɛrinɛti ye, olu ye se sɔrɔmiligaramu kɛmɛ caman/Lastaxanthin sɔrɔli cogoya ɲumanw na.
5. Fermentation Process Optimisation (Kɛnɛya kɛcogo ɲuman).
Sɛgɛsɛgɛli kɔfɛ, fermentation paramètres jɔyɔrɔ ka bon kosɛbɛ izini-skala baarakɛcogo la.
Fɛn minnu bɛ nɔ bila mɔgɔ la, olu jɔnjɔnw ye:
Kabɔn sɔrɔyɔrɔw: sukaro, lignocellulose hydrolysates, walima jirituluw
Azote sɔrɔyɔrɔw: funteni bɔlen ni peptone de ka fisa ka caya
C/N hakɛ: a hakɛ cayalenba ka teli ka karotɛni dalajɛli dɛmɛ
pH kɔlɔsili: asidi dɔɔni cogoyaw (pH 4,5–5,0 lamini na) a ka ca a la olu ka ɲi
Oksizɛni min bɛ wuli (DO) .: oksizɛni sɔrɔli bɛrɛbɛrɛ bɛ selilɛriw falenni ni anzimu baara fila bɛɛ dɛmɛ
Nin paramɛtiri ninnu faralen ɲɔgɔn kan ka ɲɛ, o bɛ se ka astaxanthin laban sɔrɔta ni baarakɛcogo sabatili bonya kosɛbɛ.
6. Industriw ka jateminɛw
Leve-based astaxanthin sɔrɔli bɛ a jiramin bɛ se ka sɛgɛsɛgɛ, min bɛ sabati, ani min bɛ se ka kɔrɔsifɛɛrɛ wɛrɛ min bɛ se ka kɛ laadala bɔli ye ka bɔ jɛgɛw la walima kemikɛlifɛnw dilanni na. Ni ɲɛtaa bɛ senna farikoloɲɛnajɛ ni fɛnɲɛnamafagalanw dilanni fɛɛrɛw la, a jirala ko astaxanthin min bɛ sɔrɔ banakisɛw la, o jɔyɔrɔ bɛna bonya ka taa a fɛ:
Dumuni ni balodɛsɛ furaw
Jilafɛnw ɲɛjibɔ furaw
Baarakɛminɛnw ni farilajidɛsɛ furaw
Ka ɲɛsin feerekɛlaw ni sannikɛlaw ma minnu bɛ ɲinini kɛjogo sabatili, sariyaw labatoli, ani musaka kɛcogo ɲuman kuntaalajan-, yezu-bɔlen astaxanthin nafama bɛ jago seko barikama di.
Miirili labanw
Ɲɛtaa minnu kɛra yezu biyoteknoloji la, olu bɛ ka astaxanthin caman tigɛli teliya la ka bɔ niche high-value compound ye ka kɛ anizini nafamafɛn nafama min bɛ se ka kɛ. Ni kokuradonni bɛ senna sɛnɛfɛnw yiriwali ni taabolo ɲuman na, o bɛna dɔ bɔ fɛn dilanni musakaw la ka taa a fɛ, ka dɔ fara a ka baarakɛcogo kan diɲɛ kɔnɔ.
